Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 3: Changes of p-ATM, p53, p21, p-Chk1, p-Chk2 and p-cdc25C protein expression levels in the kidney at 42 days of age. (Immunohistochemistry, ×400).

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Immunohistochemistry

Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 5: Changes of the mean density of p-ATM, p53, p21, p-Chk1, p-Chk2 and p-cdc25C protein expression in the kidney. Data are presented with the mean ± standard deviation (n=5×5) *P<0.05, compared with the control group **P<0.01, compared with the control group.

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Standard Deviation, Control

Journal: Oncotarget

Article Title: Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney.

doi: 10.18632/oncotarget.5934

Figure Lengend Snippet: Figure 7: Changes of ATM, p53, p21, Chk1, Chk2 and cdc25 mRNA expression levels in the kidney. Data are presented with the mean ± standard deviation (n=5) *P<0.05, compared with the control group **P<0.01, compared with the control group.

Article Snippet: Table 1: Antibodies used in immunohitochemistry Name Company Cat# Dilution p-ATM Bioss, China bs-2272R 1:100 p-Chk1 Bioss, China bs-5251R 1:100 p-Chk2 Bioss, China bs-3721R 1:100 p53 Boster, China BM0101 1:100 p21 Boster, China BA0272 1:100 p-cdc25C Bioss, China bs-3482R 1:100 p-cdc2 Boster, China BM0027 1:100 cyclinB1 Bioss, China bs-0572R 1:100 PCNA Boster, China BM0104 1:100 Oncotarget35969www.impactjournals.com/oncotarget or P < 0.01) in the 900 mg/kg groups at 14 days of age and in the three NiCl2-treated groups from 28 to 42 days of age in comparison with those in the control group.

Techniques: Expressing, Standard Deviation, Control

Journal: Current Health Sciences Journal

Article Title: P21 Immunoexpression in Actinic Keratosis and Cutaneous Carcinomas

doi: 10.12865/CHSJ.43.03.07

Figure Lengend Snippet: p21 immunostaining: A. Actinic keratosis (KIN I), x100; B. well differentiated squamous carcinoma, x100; C. moderate differentiated squamous carcinoma, x100; D. poorly differentiated squamous carcinoma, x100

Article Snippet: In this study we use a monoclonal mouse anti-human anti-p21 antibody (Biocare Medical), clone WA-1, diluted as 1:50, with microwaving in citrate buffer pH 6 as antigen retrieval.

Techniques: Immunostaining

Journal: Current Health Sciences Journal

Article Title: P21 Immunoexpression in Actinic Keratosis and Cutaneous Carcinomas

doi: 10.12865/CHSJ.43.03.07

Figure Lengend Snippet: Distribution of the actinic keratosis cases depending of p21 FSS

Article Snippet: In this study we use a monoclonal mouse anti-human anti-p21 antibody (Biocare Medical), clone WA-1, diluted as 1:50, with microwaving in citrate buffer pH 6 as antigen retrieval.

Techniques:

Journal: Current Health Sciences Journal

Article Title: P21 Immunoexpression in Actinic Keratosis and Cutaneous Carcinomas

doi: 10.12865/CHSJ.43.03.07

Figure Lengend Snippet: Distribution of the squamous carcinomas cases depending on p21 FSS

Article Snippet: In this study we use a monoclonal mouse anti-human anti-p21 antibody (Biocare Medical), clone WA-1, diluted as 1:50, with microwaving in citrate buffer pH 6 as antigen retrieval.

Techniques:

Journal: Current Health Sciences Journal

Article Title: P21 Immunoexpression in Actinic Keratosis and Cutaneous Carcinomas

doi: 10.12865/CHSJ.43.03.07

Figure Lengend Snippet: Distribution of the positive p21 carcinomas depending on differentiation degree (A) and tumor stage (B)

Article Snippet: In this study we use a monoclonal mouse anti-human anti-p21 antibody (Biocare Medical), clone WA-1, diluted as 1:50, with microwaving in citrate buffer pH 6 as antigen retrieval.

Techniques:

Journal: Journal of Biomedical Science

Article Title: MiR-155 promotes proliferation of human breast cancer MCF-7 cells through targeting tumor protein 53-induced nuclear protein 1

doi: 10.1186/1423-0127-20-79

Figure Lengend Snippet: MiR-155 negatively regulated protein expression of cleaved-caspase-3, -8, -9, and p21 in MCF-7 cells. (A) Protein expression levels of p21 and cleaved-caspase-3 after transfection with miR-155 m. (B) Protein expression levels of p21 and cleaved-caspase-3 after transfection with miR-155i. (C) Protein expression levels of cleaved-caspase-8, -9 after transfection with miR-155 m. (D) Protein expression levels of cleaved-caspase-8, -9 after transfection with miR-155i. The protein expression levels of cleaved-caspase-3, -8, -9, and p21 were detected by western blot and normalized to that of β- actin. Data are presented as mean ± SD. ** P < 0.01 and * P < 0.05 versus Con, miR-155 m NC or miR-155i NC. All experiments were repeated at least three times.

Article Snippet: After blocked 2 h with 5% milk, the PVDF membranes were then incubated with TP53INP1 (rabbit anti-human polyclonal antibody; 1:100, Santa Cruz), pro-caspase-3 (rabbit anti-human monoclonal antibody; 1:1,000, epitomics), cleaved-caspase-3 (rabbit anti-human monoclonal antibody; 1:1,000, Beyotime), caspase-8 (rabbit anti-human monoclonal antibody; pro-caspase-8: 1:1,000, cleaved-caspase-8: 1:500, Beyotime), caspase-9 (mouse anti- human monoclonal antibody; 1:1,000, Beyotime), and p21 (mouse anti- human monoclonal antibody; 1:200, Beyotime) overnight at 4°C.

Techniques: Expressing, Transfection, Western Blot